- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-groES抗体
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描述兔多克隆抗体to groES
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宿主Rabbit
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经测试应用适用于: WB, IPmore details
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种属反应性与反应: Escherichia coli
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免疫原
Full length GroES purified chromatographically from an E. coli strain overexpressing the protein.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: Whole serum -
纯度Whole antiserum
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab69823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). for colorimetric detection. 1/500 for ECL. | |
| IP | 1/200. |
靶标
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相关性GroEL and GroES are E.coli chaperones, which are homologs of the mitochondrial chaperones Hsp60 and Hsp10 respectively. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding in an ATP-dependent mechanism. Binding of substrate protein, in addition to binding of ATP, induces a conformational change that allows association of this binary complex with a separate lid structure, GroES.
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细胞定位Cytoplasmic
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数据库链接
- Entrez Gene: 959981 Escherichia coli
- SwissProt: P0A6G1 Escherichia coli
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别名
- 10 kDa chaperonin antibody
- Co-chaperonin GroES antibody
- groES protein antibody
see all
图片
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Western blot - Anti-groES antibody (ab69823)All lanes : Anti-groES antibody (ab69823) at 1/5000 dilution
Lane 1 : Molecular weight marker
Lane 2 : groES recombinant E. coli protein
Lane 3 : No lysates (empty)
Lane 4 : Lysates prepared from E.coli
Predicted band size: 10 kDa
文献
This product has been referenced in:
- Sabater-Muñoz B et al. Fitness trade-offs determine the role of the molecular chaperonin GroEL in buffering mutations. Mol Biol Evol N/A:N/A (2015). Read more (PubMed: 26116858) »
- Zhou Y & Chen WN iTRAQ-coupled 2-D LC-MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB. J Proteomics : (2011). WB ; Escherichia coli . Read more (PubMed: 21889622) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"