- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
-
产品名称Anti-groES抗体
-
描述兔多克隆抗体to groES
-
宿主Rabbit
-
经测试应用适用于: WB, IPmore details
-
种属反应性与反应: Escherichia coli
-
免疫原
Full length GroES purified chromatographically from an E. coli strain overexpressing the protein.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
存储溶液Preservative: None
Constituents: Whole serum -
纯度Whole antiserum
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab69823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). for colorimetric detection. 1/500 for ECL. | |
| IP | 1/200. |
靶标
-
相关性GroEL and GroES are E.coli chaperones, which are homologs of the mitochondrial chaperones Hsp60 and Hsp10 respectively. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding in an ATP-dependent mechanism. Binding of substrate protein, in addition to binding of ATP, induces a conformational change that allows association of this binary complex with a separate lid structure, GroES.
-
细胞定位Cytoplasmic
-
数据库链接
- Entrez Gene: 959981 Escherichia coli
- SwissProt: P0A6G1 Escherichia coli
-
别名
- 10 kDa chaperonin antibody
- Co-chaperonin GroES antibody
- groES protein antibody
see all
图片
-
Western blot - groES antibody (ab69823)All lanes : Anti-groES antibody (ab69823) at 1/5000 dilution
Lane 1 : Molecular weight marker
Lane 2 : groES recombinant E. coli protein
Lane 3 : No lysates (empty)
Lane 4 : Lysates prepared from E.coli
Predicted band size: 10 kDa
文献
This product has been referenced in:
- Sabater-Muñoz B et al. Fitness trade-offs determine the role of the molecular chaperonin GroEL in buffering mutations. Mol Biol Evol N/A:N/A (2015). Read more (PubMed: 26116858) »
- Zhou Y & Chen WN iTRAQ-coupled 2-D LC-MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB. J Proteomics : (2011). WB ; Escherichia coli . Read more (PubMed: 21889622) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"