Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (18)
Sample
Escherichia coli Cell lysate - whole cell (E. coli Deletion variants)
Specification
E. coli Deletion variants
Treatment
20 mM Nitrat, no oxygen for 16 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBSt
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 57 kDa
Exposure
35 second(s)
Positive control
Wild type Strain
Additional data
Additional Notes
Concentration recommend seems to high
Re-use of antibody worked well (tested after 1 week at 4 ¯C)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 14 2014