Highly pure (>98%) recombinant hGROg (human GRO-gamma)
形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液PBS, pH 7.4, no preservative, sterile filtered
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hGROg (100.00 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required.
ELISA:To detect hGROg by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGROg.
Western Blot:To detect hGROg by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGROg is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
功能Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
翻译后修饰N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.