Anti-GRLF1 (phospho Y1105)抗体(ab55339)

概述

  • 产品名称Anti-GRLF1 (phospho Y1105)抗体
    参阅全部 GRLF1 一抗
  • 描述
    兔多克隆抗体to GRLF1 (phospho Y1105)
  • 特异性This antibody detects endogenous levels of GRLF1 only when phosphorylated at tyrosine 1105.
  • 经测试应用适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human)(Peptide available as ab106616.)

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
  • 序列相似性Contains 4 FF domains.
    Contains 1 Rho-GAP domain.
  • 翻译后修饰Tyrosine phosphorylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHGAP35 antibody
    • GAP associated protein p190 antibody
    • Glucocorticoid receptor DNA binding factor 1 antibody
    • Glucocorticoid receptor DNA-binding factor 1 antibody
    • Glucocorticoid receptor repression factor 1 antibody
    • GRF 1 antibody
    • GRF-1 antibody
    • GRF1 antibody
    • GRLF 1 antibody
    • GRLF1 antibody
    • GRLF1_HUMAN antibody
    • KIAA1722 antibody
    • MGC10745 antibody
    • p190 A antibody
    • p190-A antibody
    • P190A antibody
    • P190A, rat, homolog of antibody
    • p190ARhoGAP antibody
    • p190RhoGAP antibody
    • Rho GAP p190A antibody
    • Rho GTPase-activating protein 35 antibody
    see all

Anti-GRLF1 (phospho Y1105) antibody 图像

  • ab55339, at 1/50 dilution, staining GRLF1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising phosphopeptide.

Anti-GRLF1 (phospho Y1105) antibody (ab55339)参考文献

ab55339 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (kidney)
Loading amount 60 µg
Specification kidney
Gel Running Conditions Reduced Denaturing (7,5%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"