Anti-GRLF1抗体(ab85950)
Key features and details
- Rabbit polyclonal to GRLF1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GRLF1抗体
参阅全部 GRLF1 一抗 -
描述
兔多克隆抗体to GRLF1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human GRLF1 aa 1200-1300.
Database link: Q9NRY4 -
阳性对照
- WB: HeLa, 293T, CT26-WT, 4T1 and Renca whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85950 was affinity purified using an epitope specific to GRLF1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 172 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 172 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor. -
序列相似性
Contains 4 FF domains.
Contains 1 Rho-GAP domain. -
翻译后修饰
Tyrosine phosphorylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2909 Human
- Entrez Gene: 232906 Mouse
- Entrez Gene: 306400 Rat
- Omim: 605277 Human
- SwissProt: Q9NRY4 Human
- SwissProt: Q91YM2 Mouse
- SwissProt: P81128 Rat
- Unigene: 509447 Human
see all -
别名
- ARHGAP35 antibody
- GAP associated protein p190 antibody
- Glucocorticoid receptor DNA binding factor 1 antibody
see all
图片
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All lanes : Anti-GRLF1 antibody (ab85950) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 172 kDa
Observed band size: 172 kDa
Exposure time: 10 seconds -
All lanes : Anti-GRLF1 antibody (ab85950) at 1/2000 dilution
Lane 1 : CT26-WT (Mouse colon carcinoma cell - Wild Type) whole cell lysate
Lane 2 : 4T1 (Mouse mammary gland cells) whole cell lysate
Lane 3 : Renca (Mouse renal carcinoma cells) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 172 kDa
Exposure time: 30 seconds -
Detection of human GRLF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85950 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab85950 尚未被引用在任何文献中。