概述

  • 产品名称
  • 描述
    兔多克隆抗体to GRK6
  • 特异性
    ab64915 detects endogenous levels of total GRK6 protein.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the N terminal of Human GRK6

  • 阳性对照
    • HeLa cells, and extracts from Jurkat, COLO205 and HUVEC cells.

性能

应用

Our Abpromise guarantee covers the use of ab64915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

靶标

  • 功能
    Specifically phosphorylates the activated forms of G protein-coupled receptors.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰
    It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing.
  • 别名
    • FLJ32135 antibody
    • G protein coupled receptor kinase 6 antibody
    • G protein coupled receptor kinase GRK6 antibody
    • G protein-coupled receptor kinase 6 antibody
    • G protein-coupled receptor kinase GRK6 antibody
    • Gprk6 antibody
    • Grk6 antibody
    • GRK6_HUMAN antibody
    see all

Anti-GRK6 antibody 图像

  • All lanes : Anti-GRK6 antibody (ab64915) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 20 kDa,26 kDa. We are unsure as to the identity of these extra bands.
  • ab64915, at a 1/500 dilution, staining Human GRK6 in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
  • IHC image of ab64915 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64915, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GRK6 antibody (ab64915)参考文献

This product has been referenced in:
  • Funk AJ  et al. Increased G protein-coupled receptor kinase (GRK) expression in the anterior cingulate cortex in schizophrenia. Schizophr Res 159:130-5 (2014). Read more (PubMed: 25153362) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
LiCor buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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