概述

  • 产品名称
  • 描述
    兔多克隆抗体to GRK6
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human GRK6.

  • 阳性对照
    • This antibody gave a positive signal in both Jurkat and K562 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab106673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Specifically phosphorylates the activated forms of G protein-coupled receptors.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰
    It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing.
  • 别名
    • FLJ32135 antibody
    • G protein coupled receptor kinase 6 antibody
    • G protein coupled receptor kinase GRK6 antibody
    • G protein-coupled receptor kinase 6 antibody
    • G protein-coupled receptor kinase GRK6 antibody
    • Gprk6 antibody
    • Grk6 antibody
    • GRK6_HUMAN antibody
    see all

图片

  • All lanes : Anti-GRK6 antibody (ab106673) at 1 µg/ml (Milk Blocking - 3%)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 112 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of ab106673 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106673, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

ab106673 has not yet been referenced specifically in any publications.

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