Anti-GRK2 (phospho S29)抗体(ab58520)

概述

  • 产品名称
    Anti-GRK2 (phospho S29)抗体
    参阅全部 GRK2 一抗
  • 描述
    兔多克隆抗体to GRK2 (phospho S29)
  • 特异性
    This antibody detects endogenous levels of GRK2 only when phosphorylated at serine 29.
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 29 (R-A-SP-K-K).

  • 阳性对照
    • IHC-P: human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.

靶标

  • 功能
    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • 组织特异性
    Expressed in peripheral blood leukocytes.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

图片

  • This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
  • Immunohistochemical analysis of adult mouse brain tissue, staining GRK2 (phospho S29) with ab58520.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100and blocked with 10% horse serum for 1 hour at 20°C;. Samples were incubated with primary antibody (1/1000 in 10% horse serum in PBS + 0.1% Triton X-100) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)

    Lane 1 : Jurkat cells treated with EGF
    Lane 2 : Jurkat cells treated with EGF with phospho peptide


    Predicted band size : 80 kDa

文献

ab58520 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (adult mouse brain)
Specification
adult mouse brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Hai-Ying Mary Cheng

Verified customer

提交于 Dec 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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