Anti-GRK1 (phospho S21)抗体(ab74033)

概述

  • 产品名称Anti-GRK1 (phospho S21)抗体
    参阅全部 GRK1 一抗
  • 描述
    兔多克隆抗体to GRK1 (phospho S21)
  • 特异性ab74033 detects endogenous levels of GRK1 only when phosphorylated at serine 21.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human GRK1 around the phosphorylation site of serine 21 (R-G-SP-F-D).

  • 阳性对照
    • COS7 cell extracts treated with TNF (20ng/ml, 5mins) and human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab74033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • 组织特异性Retinal-specific. Expressed in rods and cones cells.
  • 疾病相关Night blindness, congenital stationary, Oguchi type 2
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

Anti-GRK1 (phospho S21) antibody 图像

  • ab74033 at 1/50 dilution staining GRK1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-GRK1 (phospho S21) antibody (ab74033) at 1/500 dilution

    Lane 1 : COS7 cell extracts, treated with TNF (20ng/ml, 5mins)
    Lane 2 : COS7 cell extracts, treated with TNF (20ng/ml, 5mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-GRK1 (phospho S21) antibody (ab74033)参考文献

ab74033 has not yet been referenced specifically in any publications.

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