概述

  • 产品名称
    Anti-GRK1抗体[G8]
    参阅全部 GRK1 一抗
  • 描述
    小鼠单克隆抗体[G8] to GRK1
  • 特异性
    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).
  • 经测试应用
    适用于: ICC/IF, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cow, Dog, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • 表位
    This antibody recognizes an epitope in the C-terminal region of GRK1a.

性能

应用

Our Abpromise guarantee covers the use of ab2775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • 组织特异性
    Retinal-specific. Expressed in rods and cones cells.
  • 疾病相关
    Night blindness, congenital stationary, Oguchi type 2
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunolocalization of GRK1 in bovine retina using ab2775.

文献

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客户评价及客户问答

This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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