概述

  • 产品名称Anti-GRK1抗体[D11]
    参阅全部 GRK1 一抗
  • 描述
    小鼠单克隆抗体[D11] to GRK1
  • 特异性Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • 表位This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

性能

应用

Our Abpromise guarantee covers the use of ab2776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20 - 1/200.
IHC-Fr 1/1000.
WB 1/1000.
IP Use at an assay dependent concentration.

靶标

  • 功能Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • 组织特异性Retinal-specific. Expressed in rods and cones cells.
  • 疾病相关Night blindness, congenital stationary, Oguchi type 2
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

Anti-GRK1 antibody [D11] 图像

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunolocalization of GRK1 in bovine retina using ab2776.

Anti-GRK1 antibody [D11] (ab2776)参考文献

This product has been referenced in:
  • Sakurai K  et al. Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors. Invest Ophthalmol Vis Sci 52:6793-800 (2011). WB . Read more (PubMed: 21474765) »
  • Higgins MK  et al. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem 281:19426-32 (2006). Read more (PubMed: 16675451) »

See all 2 Publications for this product

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