Anti-GRIP1抗体(ab66755)

概述

  • 产品名称Anti-GRIP1抗体
    参阅全部 GRIP1 一抗
  • 描述
    兔多克隆抗体to GRIP1
  • 特异性This antibody is raised against glutamate receptor-interacting protein 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog
  • 免疫原

    Synthetic peptide (Human)

性能

应用

Our Abpromise guarantee covers the use of ab66755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • 序列相似性Contains 7 PDZ (DHR) domains.
  • 结构域PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • 细胞定位Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 数据库链接
  • 别名
    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all

Anti-GRIP1 antibody 图像

  • Anti-GRIP1 antibody (ab66755) at 1.25 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 117 kDa
    Observed band size : 117 kDa

Anti-GRIP1 antibody (ab66755)参考文献

ab66755 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 150 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"