概述
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产品名称GRIM19 overexpression 293T裂解液(全细胞)
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描述GRIM19 overexpression裂解液
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常规说明ab94271 is a 293T cell transfected lysate in which Human GRIM19 has been transiently over-expressed using a pCMV-GRIM19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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经测试应用适用于: WBmore details
性能
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形式Liquid
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存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
纯度Whole Cell Lysate
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研究领域
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背景Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. Tissue specificity: Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. Disease: Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Similarity: Belongs to the complex I NDUFA13 subunit family. Developmental stage: Expressed in numerous fetal tissues.
应用
Our Abpromise guarantee covers the use of ab94271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
GRIM19 overexpression 293T lysate (whole cell) 图像
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SDS-PAGE - GRIM19 293T Transfected Lysate - (positive control) (ab94271)ab94271 at 15µg/lane on an SDS-PAGE gel. -
Western blot - GRIM19 293T Transfected Lysate - (positive control) (ab94271)All lanes : Anti-GRIM19 antibody (ab88094) at 1/500 dilution
Lane 1 :GRIM19 overexpression 293T lysate (whole cell) (ab94271)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
GRIM19 overexpression 293T lysate (whole cell) (ab94271)参考文献
ab94271 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"