Anti-GRIM19抗体[6E1BH7] (ab110240)

概述

  • 产品名称
    Anti-GRIM19抗体[6E1BH7]
    参阅全部 GRIM19 一抗
  • 描述
    小鼠单克隆抗体[6E1BH7] to GRIM19
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein Human GRIM19.

  • 阳性对照
    • Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria, Human fibroblasts, HeLa cells
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

性能

应用

Our Abpromise guarantee covers the use of ab110240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 8 µg/ml. In-Cell ELISA (0.8 µg/well)
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
  • 组织特异性
    Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
  • 疾病相关
    Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
  • 序列相似性
    Belongs to the complex I NDUFA13 subunit family.
  • 发展阶段
    Expressed in numerous fetal tissues.
  • 细胞定位
    Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2700054G14Rik antibody
    • AU022060 antibody
    • B16.6 antibody
    • CDA016 antibody
    • Cell death regulatory protein antibody
    • Cell death regulatory protein GRIM-19 antibody
    • CGI-39 antibody
    • CGI39 protein antibody
    • CI-B16.6 antibody
    • Complex I-B16.6 antibody
    • FLJ58045 antibody
    • FLJ59191 antibody
    • Gene associated with retinoic and IFN-induced mortality 19 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 19 protein antibody
    • Gene associated with retinoic interferon induced mortality 19 protein antibody
    • GRIM-19 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody
    • NADH ubiquinone oxidoreductase B16.6 subunit antibody
    • NADH-ubiquinone oxidoreductase B16.6 subunit antibody
    • NDUAD_HUMAN antibody
    • NDUFA13 antibody
    • RGD1565358 antibody
    see all

图片

  • All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml

    Lane 1 : Human heart mitochondria
    Lane 2 : Bovine heart mitochondria
    Lane 3 : Rat heart mitochondria
    Lane 4 : Mouse heart mitochondria


    Predicted band size : 17 kDa
  • Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
  • HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110240 staining GRIM19 in Human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/3000 in TBS) for 1 hour and stained with DAB.

    See Abreview

文献

This product has been referenced in:
  • Kim JH  et al. GRIM-19 Restricts HCV Replication by Attenuating Intracellular Lipid Accumulation. Front Microbiol 8:576 (2017). WB . Read more (PubMed: 28443075) »
  • Srinivasan S  et al. Disruption of cytochrome c oxidase function induces the Warburg effect and metabolic reprogramming. Oncogene 35:1585-95 (2016). Read more (PubMed: 26148236) »

See all 23 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain - Cerebellum)
Specification
Brain - Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Username

Ms. Philippa Hepplewhite

Verified customer

提交于 Jun 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Isolated Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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