概述

  • 产品名称Anti-GRIK2 + GRIK3抗体
  • 描述
    兔多克隆抗体to GRIK2 + GRIK3
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Monkey
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

  • 阳性对照
    • Brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab15307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 105 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Anti-GRIK2 + GRIK3 antibody 图像

  • ab15307 staining GRIK2/3 in human brain by Immunohistochemistry (FFPE-sections).
  • ab15307 at 1/200 staining rat brain (PAG) tissue sections by IHC (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed and the tissue was then incubated with the antibody for 24 hours. A biotinylated goat polyclonal antibody was used as the secondary.

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  • Anti-GRIK2 + GRIK3 antibody (ab15307) at 1/2000 dilution + Rat brain cortex lysate at 15ug per lane

    Secondary
    Horse anti-rabbit HRP at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

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Anti-GRIK2 + GRIK3 antibody (ab15307)参考文献

This product has been referenced in:
  • Rinaldi T  et al. Elevated NMDA receptor levels and enhanced postsynaptic long-term potentiation induced by prenatal exposure to valproic acid. Proc Natl Acad Sci U S A 104:13501-6 (2007). Read more (PubMed: 17675408) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Brain Cortex)
Loading amount 15 µg
Specification Brain Cortex
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Oct 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain (PAG))
Specification brain (PAG)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
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Abcam user community

Verified customer

提交于 Feb 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"