概述

  • 产品名称Anti-GRID2抗体
    参阅全部 GRID2 一抗
  • 描述
    兔多克隆抗体to GRID2
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human GRID2.

  • 阳性对照
    • Extracts from NIH/3T3 cells. HuvEc cells.

性能

应用

Our Abpromise guarantee covers the use of ab74866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 113 kDa.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.
  • 结构域The PDZ-binding motif mediates interaction with GOPC.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • delta-2 antibody
    • GluD2 antibody
    • GLUR D2 antibody
    • GluR delta 2 antibody
    • GluR delta-2 subunit antibody
    • GLURD 2 antibody
    • GLURD2 antibody
    • Glutamate receptor delta 2 subunit antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor ionotropic delta 2 antibody
    • GRID 2 antibody
    • Grid2 antibody
    • GRID2_HUMAN antibody
    • MGC117022 antibody
    • MGC117023 antibody
    • MGC117024 antibody
    see all

Anti-GRID2 antibody 图像

  • All lanes : Anti-GRID2 antibody (ab74866) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 113 kDa
    Observed band size : 113 kDa
  • Immunofluorescence analysis of GRID2 in HuvEc cells, using ab74866, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-GRID2 antibody (ab74866)参考文献

This product has been referenced in:
  • Tang M  et al. Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors. PLoS One 7:e51433 (2012). WB ; Mouse . Read more (PubMed: 23236500) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"