参阅全部 GRHL3 一抗
WB, ELISAmore details
A region within synthetic peptide: SGVKGCLLSG FRGNETTYLR PETDLETPPV LFIPNVHFSS LQRSGGAAPS, corresponding to amino acids 428-477 of Human GRHL3
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
- 功能May function as a transcription factor.
- 组织特异性Expressed in brain, colon, pancreas, placenta and kidney. Isoform 1 is expressed in lung and tonsil. Isoform 2 is prostate-specific.
- 序列相似性Belongs to the grh/CP2 family. Grainyhead subfamily.
Information by UniProt
- Drosophila antibody
- Grainyhead like 3 antibody
- Grainyhead like protein 3 homolog antibody
Anti-GRHL3 antibody 图像
Western blot - GRHL3 antibody (ab50755)
Lane 1 : marker
Lane 2 : Anti-GRHL3 antibody (ab50755) at 0.5 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000.
Predicted band size : 70 kDa
Observed band size : ~57 kDa (why is the actual band size different from the predicted?)
Anti-GRHL3 antibody (ab50755)参考文献
has not yet been referenced specifically in any publications.
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