Anti-GRHL2抗体(ab86611)

概述

  • 产品名称Anti-GRHL2抗体
    参阅全部 GRHL2 一抗
  • 描述
    兔多克隆抗体to GRHL2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide from within a region of internal sequence amino acids 576-625 (EKIAKLYKKS KKGILVNMDD NIIEHYSNED TFILNMESMV EGFKVTLMEI) of Human GRHL2 (NP_079191)

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May function as a transcription factor.
  • 组织特异性Highly expressed in placenta, prostate, brain and kidney. Lower-level expression in a variety of epithelial tissues such as thymus, kidney, lung, salivary gland, mammary gland and digestive tract. Expressed in the cochlear.
  • 疾病相关Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28) [MIM:608641]. DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age.
  • 序列相似性Belongs to the grh/CP2 family. Grainyhead subfamily.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BOM antibody
    • Brother of mammalian grainyhead antibody
    • Deafness autosomal dominant 28 antibody
    • DFNA28 antibody
    • ECTDS antibody
    • FLJ11172 antibody
    • FLJ13782 antibody
    • Grainyhead like 2 (Drosophila) antibody
    • Grainyhead like 2 antibody
    • Grainyhead like protein 2 homolog antibody
    • Grainyhead like transcription factor 2 antibody
    • Grainyhead-like protein 2 homolog antibody
    • GRHL 2 antibody
    • GRHL2 antibody
    • GRHL2_HUMAN antibody
    • MGC149294 antibody
    • MGC149295 antibody
    • RGD1561191 antibody
    • TFCP2L3 antibody
    • Transcription factor CP2 like 3 antibody
    • Transcription factor CP2-like 3 antibody
    see all

Anti-GRHL2 antibody 图像

  • Anti-GRHL2 antibody (ab86611) at 1 µg/ml (5% skim milk/ PBS buffer) + 721_B cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 35,66 kDa (why is the actual band size different from the predicted?)
  • ab86611 staining GRHL2 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-GRHL2 antibody (ab86611)参考文献

ab86611 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryo)
Antigen retrieval step Heat mediated
Permeabilization Yes - Triton
Specification embryo
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Jun 02 2016

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