概述

  • 产品名称Anti-GRF2抗体
    参阅全部 GRF2 一抗
  • 描述
    兔多克隆抗体to GRF2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Chimpanzee, Rhesus monkey, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of human GRF2 (NP_005303.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab86224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 121 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
    • 组织特异性Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
    • 序列相似性Contains 1 N-terminal Ras-GEF domain.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • 数据库链接
    • 形式It localizes to cytoplasm and endosomes.
    • 别名
      • C3G antibody
      • C3G protein antibody
      • CRK SH3 binding GNRP antibody
      • CRK SH3-binding GNRP antibody
      • DKFZp781P1719 antibody
      • GRF 2 antibody
      • GRF2 antibody
      • Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody
      • Guanine nucleotide releasing factor 2 antibody
      • Guanine nucleotide-releasing factor 2 antibody
      • OTTHUMP00000064558 antibody
      • Protein C3G antibody
      • Rap guanine nucleotide exchange factor (GEF) 1 antibody
      • Rap guanine nucleotide exchange factor 1 antibody
      • RAPGEF 1 antibody
      • RAPGEF1 antibody
      • RPGF1_HUMAN antibody
      see all

    Anti-GRF2 antibody 图像

    • All lanes : Anti-GRF2 antibody (ab86224) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 121 kDa


      Exposure time : 3 minutes
    • Detection of human GRF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86224 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-GRF2 antibody (ab86224)参考文献

    ab86224 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"