Anti-GRC5 / PHF2抗体- Carboxyterminal end (ab65771)

概述

  • 产品名称
    Anti-GRC5 / PHF2抗体- Carboxyterminal end
    参阅全部 GRC5 / PHF2 一抗
  • 描述
    兔多克隆抗体to GRC5 / PHF2 - Carboxyterminal end
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

  • 阳性对照
    • Nuclear extracts of HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab65771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
IP Use at an assay dependent dilution.

靶标

  • 功能
    Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
  • 组织特异性
    Widely expressed, including in liver (at protein level).
  • 序列相似性
    Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • 结构域
    The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
  • 翻译后修饰
    Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP-35 antibody
    • GRC5 antibody
    • JHDM1E antibody
    • Jumonji C domain containing histone demethylase 1E antibody
    • Lysine-specific demethylase PHF2 antibody
    • PHD finger protein 2 antibody
    • Phf2 antibody
    • PHF2_HUMAN antibody
    see all

图片

  • Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.

    Predicted band size : 121 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

文献

ab65771 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 109...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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