Anti-GRC5 / PHF2抗体(ab124434)

概述

  • 产品名称Anti-GRC5 / PHF2抗体
    参阅全部 GRC5 / PHF2 一抗
  • 描述
    兔多克隆抗体to GRC5 / PHF2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human GRC5/ PHF2.

  • 阳性对照
    • This antibody gave a positive signal in both Human and Mouse Liver tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab124434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
  • 组织特异性Widely expressed, including in liver (at protein level).
  • 序列相似性Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • 结构域The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
  • 翻译后修饰Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP-35 antibody
    • GRC5 antibody
    • JHDM1E antibody
    • Jumonji C domain containing histone demethylase 1E antibody
    • Lysine-specific demethylase PHF2 antibody
    • PHD finger protein 2 antibody
    • Phf2 antibody
    • PHF2_HUMAN antibody
    see all

Anti-GRC5 / PHF2 antibody 图像

  • All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-GRC5 / PHF2 antibody (ab124434)参考文献

ab124434 has not yet been referenced specifically in any publications.

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