Anti-GRC5 / PHF2抗体(ab123507)

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概述

  • 产品名称
    Anti-GRC5 / PHF2抗体
    参阅全部 GRC5 / PHF2 一抗
  • 描述
    兔多克隆抗体to GRC5 / PHF2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human GRC5/ PHF2 using the numbering given in entry NP_005383.3

  • 阳性对照
    • HeLa and 293T whole cell cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab123507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
  • 应用说明
    Is unsuitable for IP.

    • 靶标

    • 功能
      Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
    • 组织特异性
      Widely expressed, including in liver (at protein level).
    • 序列相似性
      Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
      Contains 1 JmjC domain.
      Contains 1 PHD-type zinc finger.
    • 结构域
      The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
    • 翻译后修饰
      Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
    • 细胞定位
      Nucleus > nucleolus.
    • Information by UniProt
    • 数据库链接
      • 别名
        • CENP-35 antibody
        • GRC5 antibody
        • JHDM1E antibody
        • Jumonji C domain containing histone demethylase 1E antibody
        • Lysine-specific demethylase PHF2 antibody
        • PHD finger protein 2 antibody
        • Phf2 antibody
        • PHF2_HUMAN antibody
        see all

      图片

      • Western blot - Anti-GRC5 / PHF2 antibody (ab123507)
        Western blot - Anti-GRC5 / PHF2 antibody (ab123507)
        All lanes : Anti-GRC5 / PHF2 antibody (ab123507) at 0.1 µg/ml

        Lane 1 : Whole cell lysate from HeLa at 15 µg
        Lane 2 : Whole cell lysate from HeLa at 50 µg
        Lane 3 : Whole cell lysate from 293T at 50 µg
        Lane 4 : Whole cell lysate from Jurkat at 50 µg

        Developed using the ECL technique.

        Predicted band size: 120 kDa


        Exposure time: 30 seconds

      实验方案

      文献

      ab123507 has not yet been referenced specifically in any publications.

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