Anti-GRC5 / PHF2抗体(ab123507)

概述

• 产品名称
  Anti-GRC5 / PHF2抗体
  参阅全部 GRC5 / PHF2 一抗
• 描述
  兔多克隆抗体to GRC5 / PHF2
• 经测试应用
  适用于: WBmore details
  不适用于: IP
• 种属反应性
  与反应: Human
  预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
• 免疫原

  Synthetic peptide, corresponding to a region within amino acids 650-700 of Human GRC5/ PHF2 using the numbering given in entry NP_005383.3

• 阳性对照
  • HeLa and 293T whole cell cell lysates

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  Preservative: 0.09% Sodium azide
  Constituent: 99% Tris citrate/phosphate
  Note: pH 7 to 8
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Jumonji containing proteins

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HeLa whole cell lysate (ab29545)
  • 293T whole cell lysate (ab95494)

应用

靶标

• 功能
  Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
• 组织特异性
  Widely expressed, including in liver (at protein level).
• 序列相似性
  Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
  Contains 1 JmjC domain.
  Contains 1 PHD-type zinc finger.
• 结构域
  The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
• 翻译后修饰
  Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
• 细胞定位
  Nucleus > nucleolus.

• Target information above from: UniProt accession O75151 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 5253 Human
  • Omim: 604351 Human
  • SwissProt: O75151 Human
  • Unigene: 211441 Human
• 别名
  • CENP-35 antibody
  • GRC5 antibody
  • JHDM1E antibody

  • Jumonji C domain containing histone demethylase 1E antibody
  • Lysine-specific demethylase PHF2 antibody
  • PHD finger protein 2 antibody
  • Phf2 antibody
  • PHF2_HUMAN antibody

  see all

实验方案

• Western blot protocols