Anti-GRB2抗体[Y301] (ab32111)


  • 产品名称Anti-GRB2抗体[Y301]
    参阅全部 GRB2 一抗
  • 描述
    兔单克隆抗体[Y301] to GRB2
  • 特异性ab32111 recognises GRB2. This antibody can also detect the splice isoform GRB3-3 of GRB2.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the N terminus of human GRB2

  • 阳性对照
    • Human breast carcinoma, NIH3T3 cells, PC12 cells, HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • 序列相似性Belongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • 结构域The SH3 domains mediate interaction with RALGPS1 and SHB.
    • 细胞定位Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Anti-GRB2 antibody [Y301] 图像

    • All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/2000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : HeLa cell lysate

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111.
    • Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
    • Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32111, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-GRB2 antibody [Y301] (ab32111)参考文献

    ab32111 has not yet been referenced specifically in any publications.

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    The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but ...

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    We used a heat mediated antigen retrieval method with sodium citrate, pH. 6.0.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Raji)
    Loading amount 40 µg
    Specification Raji
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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    提交于 Sep 11 2009