概述

  • 产品名称Anti-GRB2抗体[GRB-232]
    参阅全部 GRB2 一抗
  • 描述
    小鼠单克隆抗体[GRB-232] to GRB2
  • 特异性Reacts specifically with an epitope in the C-terminal region of Grb2 (a.a. 200-217).
  • 经测试应用适用于: IP, WB, ELISA, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    GQTGMFPRNYVTPVNRNV

    conjugated to KLH, corresponding to C terminal amino acids 200-217 of Human GRB2

  • 阳性对照
    • Whole extract of cultured human acute T cell leukemia Jurkat cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab49876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • 序列相似性Belongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • 结构域The SH3 domains mediate interaction with RALGPS1 and SHB.
    • 细胞定位Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Anti-GRB2 antibody [GRB-232] (ab49876)参考文献

    This product has been referenced in:
    • Fritsche L  et al. Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907. Am J Physiol Endocrinol Metab 300:E824-36 (2011). IP ; Human . Read more (PubMed: 21098738) »
    • Chentouf M  et al. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets. PLoS One 6:e22954 (2011). WB ; Rat . Read more (PubMed: 21826222) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (mouse D3 embryonic stem cells)
    Permeabilization Yes - triton 0.1%
    Specification mouse D3 embryonic stem cells
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 15°C
    Fixative Paraformaldehyde
    Username

    Miss. Fabiana Luise

    Verified customer

    提交于 Nov 30 2015

    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells D3)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 1e+007 cells
    Specification mouse embryonic stem cells D3
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 18 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (Hela cell subcellular fractionation)
    Loading amount 10 µg
    Specification Hela cell subcellular fractionation
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 17 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"