Anti-GRB10抗体(ab125583)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GRB10
  • 特异性
    At least three isoforms of GRB10 are known to exist; ab125583 will detect all three isoforms.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 17 amino acids near the C terminus of Human GRB10 (NP_005302).

  • 阳性对照
    • SK-N-SH cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab125583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa.

靶标

  • 功能
    Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • 组织特异性
    Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • 序列相似性
    Belongs to the GRB7/10/14 family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 SH2 domain.
  • 结构域
    The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
  • 翻译后修饰
    phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
  • 细胞定位
    Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • grb 10 antibody
    • GRB IR antibody
    • grb-10 antibody
    • GRB10 adapter protein antibody
    • GRB10 adaptor protein antibody
    • GRB10 antibody
    • GRB10_HUMAN antibody
    • GRBIR antibody
    • Growth factor receptor bound protein 10 antibody
    • Growth factor receptor-bound protein 10 antibody
    • Insulin receptor binding protein antibody
    • Insulin receptor binding protein GRB IR antibody
    • Insulin receptor-binding protein Grb-IR antibody
    • IRBP antibody
    • KIAA0207 antibody
    • Maternally expressed gene 1 antibody
    • MEG1 antibody
    • RSS antibody
    see all

图片

  • All lanes : Anti-GRB10 antibody (ab125583) at 1 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 67 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Lane 1 : Anti-GRB10 antibody (ab125583) at 1 µg/ml
    Lane 2 : Anti-GRB10 antibody (ab125583) at 2 µg/ml

    Lane 1 : SK-N-SH cell lysate
    Lane 2 : SK-N-SH cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 67 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunofluorescence of GRB10 in rat brain cells with ab125583 at 20 ug/mL.

文献

ab125583 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 14 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Jan 20 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Miss. Rebecca Atkinson-Dell

Verified customer

提交于 Nov 18 2015

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2014

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