Anti-GRASP65抗体[4F8] (ab117932)

概述

  • 产品名称Anti-GRASP65抗体[4F8]
    参阅全部 GRASP65 一抗
  • 描述
    小鼠单克隆抗体[4F8] to GRASP65
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human GRASP65 produced in HEK293T cells (NP_114105).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY GRASP65 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
  • 常规说明Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab117932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100.

靶标

  • 功能Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
  • 序列相似性Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • 翻译后修饰Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
  • 细胞定位Golgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23443 antibody
    • Golgi peripheral membrane protein p65 antibody
    • Golgi phosphoprotein 5 antibody
    • Golgi reassembly and stacking protein 1 antibody
    • Golgi reassembly and stacking protein 65 kDa antibody
    • Golgi reassembly and stacking protein, 65-kD antibody
    • Golgi reassembly stacking protein 1 65kDa antibody
    • Golgi reassembly stacking protein 1 antibody
    • Golgi reassembly stacking protein of 65 kDa antibody
    • Golgi reassembly-stacking protein 1 antibody
    • Golgi reassembly-stacking protein of 65 kDa antibody
    • GOLPH 5 antibody
    • GOLPH5 antibody
    • Gorasp 1 antibody
    • GORASP1 antibody
    • GORS1_HUMAN antibody
    • GRASP 65 antibody
    • GRASP65 antibody
    • MGC118894 antibody
    • MGC118897 antibody
    • P65 antibody
    see all

Anti-GRASP65 antibody [4F8] 图像

  • ab117932 at 1/100 dilution staining GRASP65 in COS7 cells transiently transfected by pCMV6-ENTRY GRASP65 by Immunofluorescence.
  • All lanes : Anti-GRASP65 antibody [4F8] (ab117932) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GRASP65 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200828)

Anti-GRASP65 antibody [4F8] (ab117932)参考文献

ab117932 has not yet been referenced specifically in any publications.

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