Anti-Granzyme K抗体[GM-24C3] (ab3771)

概述

  • 产品名称Anti-Granzyme K抗体[GM-24C3]
    参阅全部 Granzyme K 一抗
  • 描述
    小鼠单克隆抗体[GM-24C3] to Granzyme K
  • 特异性This antibody recognises Granzyme K transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. It does not cross react with Granzyme A. Specificity is routinely tested by flow cytometry on BOSC cells transiently transfected with a Granzyme K expression vector.
  • 经测试应用适用于: ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Granzyme K cDNA (see relevance text).

  • 常规说明


    Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. To date, five human granzymes (A, B, H, K,M) have been described at the molecular genetic level. Human granzyme K (GZMK) is a 28 kD aserine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. To which extent human granzyme K plays a role in the induction of apoptosis in the target cells remains to be evaluated. However, granzyme K purified from a rat large granular lymphoma cell line (RNK-16)has been shown to induce apoptosis in vitro. High mRNA levels of granzyme K are detected inactivated T cells and NK cells but are absent in normal tissues that do not contain high numbers of these cells. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. To date, five human granzymes (A, B, H, K,M) have been described at the molecular genetic level. Human granzyme K (GZMK) is a 28 kD aserine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. To which extent human granzyme K plays a role in the induction of apoptosis in the target cells remains to be evaluated. However, granzyme K purified from a rat large granular lymphoma cell line (RNK-16)has been shown to induce apoptosis in vitro. High mRNA levels of granzyme K are detected inactivated T cells and NK cells but are absent in normal tissues that do not contain high numbers of these cells. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.
  • 克隆单克隆
  • 克隆编号GM-24C3
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/200 - 1/400.
Flow Cyt Use 1.2µg for 106 cells.

靶标

  • 组织特异性Expressed in lung, spleen, thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Secreted. Cytoplasmic granule.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fragmentin 3 antibody
    • Fragmentin-3 antibody
    • Fragmentin3 antibody
    • GRAK_HUMAN antibody
    • Granzyme 3 antibody
    • granzyme K (granzyme 3; tryptase II) antibody
    • granzyme K (serine protease, granzyme 3; tryptase II) antibody
    • Granzyme K antibody
    • Granzyme K precursor antibody
    • Granzyme-3 antibody
    • Granzyme3 antibody
    • GranzymeK antibody
    • GZMK antibody
    • NK TRYP 2 antibody
    • NK TRYP2 antibody
    • NK tryptase 2 antibody
    • NK-TRYP-2 antibody
    • NK-tryptase-2 antibody
    • NKTRYP2 antibody
    • Serine protease granzyme 3 antibody
    • TRYP 2 antibody
    • TRYP2 antibody
    • Tryptase II antibody
    • TryptaseII antibody
    see all

Anti-Granzyme K antibody [GM-24C3] (ab3771)参考文献

ab3771 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Unfortunately, there was not much information I could find regarding suitable negative controls for these products, but I did manage to find information on positive controls in Swiss Prot. ab3771 - expression can be...

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Thank you for your enquiry. The three antibodies against granzyme K (ab3771, ab2615, and ab26155) will likely recognize different epitopes and have different affinities for the protein however this has not been confirmed. All three have been tested...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"