兔多克隆抗体to Granzyme K
Synthetic peptide derived from the internal region of human Granzyme K.
- Human lung carcinoma tissue. NIH/3T3 cells. Extracts from Jurkat cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
||1/50 - 1/100.
||1/500 - 1/1000.
Expressed in lung, spleen, thymus and peripheral blood leukocytes.
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Secreted. Cytoplasmic granule.
Information by UniProt
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Anti-Granzyme K antibody 图像
Western blot - Granzyme K antibody (ab69884)
All lanes :
Anti-Granzyme K antibody (ab69884) at 1/500 dilutionLane 1 :
Extracts from Jurkat cellsLane 2 :
Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.Predicted band size :
29 kDaObserved band size :
34 kDa (why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Granzyme K antibody (ab69884)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab69884 at 1/50 dilution in the presence or absence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Granzyme K antibody (ab69884)
Immunofluorescence analysis of NIH/3T3 cells using ab69884 at 1/500 dilution in the presence or absence of the immunising peptide.
Anti-Granzyme K antibody (ab69884)参考文献
has not yet been referenced specifically in any publications.
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