概述

  • 产品名称Anti-Granzyme H抗体
    参阅全部 Granzyme H 一抗
  • 描述
    兔多克隆抗体to Granzyme H
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cat
  • 免疫原

    Synthetic peptide corresponding to Human Granzyme H aa 1-50 (N terminal).
    Sequence:

    MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCG

  • 阳性对照
    • Jurkat Cell Lysate, human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab58852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

靶标

  • 功能Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
  • 组织特异性Constituvely expressed in NK cells.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cathepsin G like 2 antibody
    • Cathepsin G-like 2 antibody
    • CCP X antibody
    • CCP-X antibody
    • CGL 2 antibody
    • CGL2 antibody
    • CSP C antibody
    • CSP-C antibody
    • CTLA1 antibody
    • CTSGL2 antibody
    • Cytotoxic serine protease C antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase antibody
    • Cytotoxic T-lymphocyte proteinase antibody
    • EC 3.4.21.- antibody
    • GRAH_HUMAN antibody
    • Granzyme H (cathepsin G-like 2, protein h-CCPX) antibody
    • Granzyme H antibody
    • GZMH antibody
    • Protein h CCPX antibody
    see all

Anti-Granzyme H antibody 图像

  • Anti-Granzyme H antibody (ab58852) at 2.5 µg/ml + Jurkat Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 27 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Granzyme H with ab58852 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Granzyme H antibody (ab58852)参考文献

ab58852 has not yet been referenced specifically in any publications.

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