Granzyme B overexpression 293T裂解液(全细胞) (ab94040)

概述

  • 产品名称
    Granzyme B overexpression 293T裂解液(全细胞)
    参阅全部 Granzyme B 细胞裂解液
  • 描述
    Granzyme B overexpression裂解液
  • 常规说明
    ab94040 is a 293T cell transfected lysate in which Human Granzyme B has been transiently over-expressed using a pCMV-Granzyme B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用
    适用于: WBmore details

性能

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应用

Our Abpromise guarantee covers the use of ab94040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

图片

  • ab94040 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Granzyme B antibody (ab89415) at 1/500 dilution

    Lane 1 : Granzyme B 293T Transfected Lysate - Positive Control (ab94040)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

文献

ab94040 has not yet been referenced specifically in any publications.

客户评价及客户问答

We determined overexpression of GZMB protein by Western Blot.

However, whether the expressed form is active or inactive has not been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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