The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/10 - 1/100. Membrane permeabilization is required for flow cytometry. The use of LeucopermTM is recommended for this purpose. Use 10µl of the suggested working dilution to label 106 cells or 100ul whole blood.
IHC-Fr: Use at an assay dependent dilution. Hsi, B-L. et al. (1986)
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
Demotte N et al. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice. Cancer Res70:7476-88 (2010).
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