重组Anti-Granzyme B抗体[EPR8260] (ab134933)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8260] to Granzyme B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Granzyme B抗体[EPR8260]
参阅全部 Granzyme B 一抗 -
描述
兔单克隆抗体[EPR8260] to Granzyme B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human Hodgkins lymphoma and Human tonsil tissues WB: KARPAS-299, SR whole cell lysates; Flow cyt: No-GFP-CD16.NK-92 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8260 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
- Alexa Fluor® 647 Anti-Granzyme B antibody [EPR8260] (ab225185)
- Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
- Alexa Fluor® 555 Anti-Granzyme B antibody [EPR8260] (ab280851)
- Anti-Granzyme B antibody [EPR8260] - Mouse IgG1 (Chimeric) (ab283315)
- Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) (ab289888)
- Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)
- Alexa Fluor® 568 Anti-Granzyme B antibody [EPR8260] (ab312793)
- Alexa Fluor® 488 Anti-Granzyme B antibody [EPR8260] (ab315854)
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 28 kDa.
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IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
Flow Cyt |
1/100.
|
说明 |
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WB
1/1000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
Flow Cyt
1/100. |
靶标
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功能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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数据库链接
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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别名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
图片
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Flow cytometric analysis of 4% paraformaldehyde fixed No-GFP-CD16.NK-92 Human Natural Killer cells labeling Granzyme B with ab134933 at 1/100 dilution (Red) compared with an Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG Alexa Fluor® 488 (ab150081), at 1/5000 dilution was used as the secondary antibody.
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All lanes : Anti-Granzyme B antibody [EPR8260] (ab134933) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : SR (human pleural effusion lymphoblast, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 48 secondsNegative control: Jurkat (PMID:18437383, 25168906).
Blocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Tissue Microarrays stained for "Anti-Granzyme B antibody [EPR8260]” using "ab134933" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab134933 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab134933 被引用在 14 文献中.
- Zhu J et al. Photothermal Nano-Vaccine Promoting Antigen Presentation and Dendritic Cells Infiltration for Enhanced Immunotherapy of Melanoma via Transdermal Microneedles Delivery. Research (Wash D C) 2022:9816272 (2022). PubMed: 36157510
- Kemper K et al. Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models. Life Sci Alliance 5:N/A (2022). PubMed: 36271507
- Lopez DC et al. Multi-spectral immunofluorescence evaluation of the myeloid, T cell, and natural killer cell tumor immune microenvironment in chordoma may guide immunotherapeutic strategies. Front Oncol 12:1012058 (2022). PubMed: 36338744
- Liu D et al. A Novel Her2/VEGFR2/CD3 trispecific antibody with an optimal structural design showed improved T-cell-redirecting antitumor efficacy. Theranostics 12:7788-7803 (2022). PubMed: 36451856
- Jiang Y et al. Hypoxia enhances the production and antitumor effect of exosomes derived from natural killer cells. Ann Transl Med 9:473 (2021). PubMed: 33850870