Anti-Granzyme B抗体[B18.1.] (FITC) (ab17929)

概述

  • 产品名称Anti-Granzyme B抗体[B18.1.] (FITC)
    参阅全部 Granzyme B 一抗
  • 描述
    小鼠单克隆抗体[B18.1.] to Granzyme B (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Recognizes human granzyme B.
  • 经测试应用适用于: ICC, Flow Cyt, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 7-227 of Human Granzyme B.

  • 常规说明1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.

性能

应用

Our Abpromise guarantee covers the use of ab17929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C11 antibody
    • Cathepsin G like 1 antibody
    • Cathepsin G-like 1 antibody
    • CCPI antibody
    • CGL 1 antibody
    • CGL1 antibody
    • CSP B antibody
    • CSPB antibody
    • CTLA-1 antibody
    • CTLA1 antibody
    • CTSGL1 antibody
    • Cytotoxic serine protease B antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase 2 antibody
    • Cytotoxic T-lymphocyte proteinase 2 antibody
    • Fragmentin 2 antibody
    • Fragmentin-2 antibody
    • GRAB_HUMAN antibody
    • Granzyme 2 antibody
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
    • Granzyme B antibody
    • Granzyme-2 antibody
    • GranzymeB antibody
    • GRB antibody
    • Gzmb antibody
    • Hlp antibody
    • Human lymphocyte protein antibody
    • Lymphocyte protease antibody
    • Protease, serine, B antibody
    • SECT antibody
    • T cell serine protease 1-3E antibody
    • T-cell serine protease 1-3E antibody
    see all

Anti-Granzyme B antibody [B18.1.] (FITC) 图像

  • Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control.
    Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg of FITC- labelled ab17929 antibody. After washing in FACS buffer, cells were anlyzed by flow cytometry.

  • Detection of granzyme B in a human CD8+ T cell clone.
    Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of the non-conjugated form of ab17929 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.

  • Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species.

    Lane 1: 40 mg of lysate
    Lane 2: 4 mg of lysate
    Lane 3: 0.4 mg of lysate

    Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B non-conjugated form of antibody ab17929 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Anti-Granzyme B antibody [B18.1.] (FITC) (ab17929)参考文献

This product has been referenced in:
  • Jovanovic DV  et al. CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages. J Rheumatol 38:419-28 (2011). Flow Cyt ; Human . Read more (PubMed: 21159825) »

See 1 Publication for this product

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