The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.
Use at an assay dependent concentration. PubMed: 24475019
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
Tsai YG et al. CD8? Treg cells associated with decreasing disease activity after intravenous methylprednisolone pulse therapy in lupus nephritis with heavy proteinuria. PLoS One9:e81344 (2014).
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