概述

  • 产品名称Anti-Granzyme B抗体
    参阅全部 Granzyme B 一抗
  • 描述
    兔多克隆抗体to Granzyme B
  • 特异性Detects endogenous levels of total Granzyme B protein. Due to sequence homology this antibody is predicted to cross-react with granzyme H.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • NIH/3T3 cell extract or Human breast carcinoma tissue IF/ICC - methanol fixed MEF1 cells.

性能

应用

Our Abpromise guarantee covers the use of ab53097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 50 - 100 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C11 antibody
    • Cathepsin G like 1 antibody
    • Cathepsin G-like 1 antibody
    • CCPI antibody
    • CGL 1 antibody
    • CGL1 antibody
    • CSP B antibody
    • CSPB antibody
    • CTLA-1 antibody
    • CTLA1 antibody
    • CTSGL1 antibody
    • Cytotoxic serine protease B antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase 2 antibody
    • Cytotoxic T-lymphocyte proteinase 2 antibody
    • Fragmentin 2 antibody
    • Fragmentin-2 antibody
    • GRAB_HUMAN antibody
    • Granzyme 2 antibody
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
    • Granzyme B antibody
    • Granzyme-2 antibody
    • GranzymeB antibody
    • GRB antibody
    • Gzmb antibody
    • Hlp antibody
    • Human lymphocyte protein antibody
    • Lymphocyte protease antibody
    • Protease, serine, B antibody
    • SECT antibody
    • T cell serine protease 1-3E antibody
    • T-cell serine protease 1-3E antibody
    see all

Anti-Granzyme B antibody 图像

  • ICC/IF image of ab53097 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.
  • All lanes : Anti-Granzyme B antibody (ab53097) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunizing peptide


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-Granzyme B antibody (ab53097)参考文献

This product has been referenced in:
  • Mohamedali KA  et al. The functionalized human serine protease granzyme B/VEGF121 targets tumor vasculature and ablates tumor growth. Mol Cancer Ther 12:2055-66 (2013). ICC/IF . Read more (PubMed: 23858102) »
  • Chaitanya GV  et al. Granzyme-b is involved in mediating post-ischemic neuronal death during focal cerebral ischemia in rat model. Neuroscience 165:1203-16 (2010). WB ; Rat . Read more (PubMed: 19895873) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (canine sarcoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification canine sarcoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
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提交于 Dec 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (B cell lymphoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification B cell lymphoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 18 2015

Thank you for contacting us.

We do not have this type of clinical data as our products are for in vitro use. A literature search should be able to provide you with this kind of information.


I hope this information is helpful ...

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No problem. I hope your experiment goes well. The immunogen for the Anti-Granzyme B antibody (ab53097) is from the N-terminal region of the human Granzyme B. I hope this information helps, if you have any further questions, please don't hesitate ...

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I am very pleased to hear you would like to accept our offer and test ab53097 with ELISA. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ELISA with ab53097 and include thi...

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Thank you for contacting us. We have a few antibodies in our catalogue which may be of interest to you. Unfortunately none have been tested for application with ELISA with the native human protein. However, Anti-Granzyme B antibody (ab53097) ha...

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Thanks for your inquiry. Based on the BLAST, the immunogen homology is 100% with pig Granzyme B. Please feel free to contact me if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"