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Synthetic peptide (Human)
Our Abpromise guarantee covers the use of ab53097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use a concentration of 50 - 100 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab53097 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.