Anti-Granulocytes抗体[HIS48] (FITC) (ab93549)

概述

  • 产品名称
    Anti-Granulocytes抗体[HIS48] (FITC)
    参阅全部 Granulocytes 一抗
  • 描述
    小鼠单克隆抗体[HIS48] to Granulocytes (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    Reacts with a molecule expressed on the surface of all rat granulocytes. The antigen recognized by ab93549 is also expressed by erythroid lineage cells during their developmental stages in the bone marrow.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • Rat splenocytes
  • 常规说明


    Clone HIS48 is commonly used as a phenotypic marker for rat granulocytes.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 500mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 纯度
    Purified IgM
  • Primary antibody说明
    Clone HIS48 is commonly used as a phenotypic marker for rat granulocytes.
  • 克隆
    单克隆
  • 克隆编号
    HIS48
  • 同种型
    IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.25µg for 105-8 cells.

Use 0.25µg for 105 - 108 cells in a final volume of 100 µl.





ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性
    Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

文献

This product has been referenced in:
  • Rhodes NP  et al. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res 37:481-8 (1997). Read more (PubMed: 9407296) »
  • Badger DA  et al. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol 141:507-19 (1996). Read more (PubMed: 8975775) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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