Anti-Granulin抗体[EPR3781] (ab108608)


  • 产品名称Anti-Granulin抗体[EPR3781]
    参阅全部 Granulin 一抗
  • 描述
    兔单克隆抗体[EPR3781] to Granulin
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Granulin.

  • 阳性对照
    • A431, 293T, MCF7, HeLa, Human fetal lung and fetal kidney lysates. Human kidney tissue. IF: Malme-3M cell line
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab108608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 55, 75 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
      Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
    • 组织特异性In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
    • 疾病相关Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
    • 序列相似性Belongs to the granulin family.
    • 翻译后修饰Granulins are disulfide bridged.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acrogranin antibody
      • CLN11 antibody
      • GEP antibody
      • GP88 antibody
      • Granulin A antibody
      • Granulin B antibody
      • Granulin C antibody
      • Granulin D antibody
      • Granulin E antibody
      • Granulin epithelin antibody
      • Granulin F antibody
      • Granulin G antibody
      • Granulin-7 antibody
      • Granulins antibody
      • GRN antibody
      • GRN_HUMAN antibody
      • PC cell derived growth factor antibody
      • PCDGF antibody
      • PEPI antibody
      • PGRN antibody
      • Proepithelin antibody
      • Progranulin antibody
      see all

    Anti-Granulin antibody [EPR3781] 图像

    • All lanes : Anti-Granulin antibody [EPR3781] (ab108608) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Human fetal lung tissue lysate
      Lane 6 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 64 kDa
      Observed band size : 55,75 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108608 at 1/100.
    • ICC/IF image of ab108608 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108608, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Granulin antibody [EPR3781] (ab108608)参考文献

    This product has been referenced in:
    • Satoh J  et al. PLD3 is accumulated on neuritic plaques in Alzheimer's disease brains. Alzheimers Res Ther 6:70 (2014). Read more (PubMed: 25478031) »
    • Alquézar C  et al. PGRN haploinsufficiency increased Wnt5a signaling in peripheral cells from frontotemporal lobar degeneration-progranulin mutation carriers. Neurobiol Aging N/A:N/A (2013). Read more (PubMed: 24139281) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    For ab108608, the immunogen is within a.a. 40 - 60.

    For ab140653, the imuunogen is within a.a. 570 - 590.

    For ab53880, the immunogen is considered proprietary from the lab, but a BLAST search w...

    Read More

    Gracias por contactarnos de nuevo.

    Sin duda en esta ocasión los resultados obtenidos son más satisfactorios, y corresponden con los obtenidos por nosotros en el laboratorio, en los que se aprecia una banda a 55KDa correspondient...

    Read More