兔多克隆抗体to Grainyhead-like蛋白1 homolog
Recombinant fragment, corresponding to a region within amino acids 1-208 of Human Grainyhead-like protein 1 homolog.
NT2D1, U-87 MG and MCF7 cells and whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 1% BSA, 20% Glycerol
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Immunogen affinity purified
ab111582 is purified by antigen affinity chromatography.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
1/100 - 1/500.
Transcription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation (By similarity). Binds and transactivates the EN1 promoter.
Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Highly expressed in placental JEG-3 cells and at very low levels in non-steroidogenic cells. No expression detected in adrenal NCI-H295A cells.
Belongs to the grh/CP2 family. Grainyhead subfamily.
Expressed in fetal liver and brain.
Information by UniProt
Grainyhead-like protein 1 homolog antibody
Western blot - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)
Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 70 kDa 7.5% SDS-PAGE.
Immunocytochemistry/ Immunofluorescence - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)
ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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