概述

  • 产品名称Anti-GPT2抗体
    参阅全部 GPT2 一抗
  • 描述
    兔多克隆抗体to GPT2
  • 特异性This antibody may cross-react with GPT1.
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 360-409 (HPEIKGQLVK LLSVRLCPPV SGQAAMDIVV NPPVAGEESF EQFSREKESV) of Human GPT2 (NP_597700).

  • 阳性对照
    • Fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
  • 组织特异性Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
  • 通路Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • 序列相似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAT2 antibody
    • Alanine aminotransferase 2 antibody
    • ALAT2_HUMAN antibody
    • ALT2 antibody
    • Glutamate pyruvate transaminase 2 antibody
    • Glutamic alanine transaminase 2 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) 2 antibody
    • Glutamic pyruvic transaminase 2 antibody
    • Glutamic--alanine transaminase 2 antibody
    • Glutamic--pyruvic transaminase 2 antibody
    • GPT 2 antibody
    • gpt2 antibody
    see all

Anti-GPT2 antibody 图像

  • Anti-GPT2 antibody (ab80947) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab80947 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80947, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPT2 antibody (ab80947)参考文献

ab80947 has not yet been referenced specifically in any publications.

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