概述

  • 产品名称Anti-GPT2抗体
    参阅全部 GPT2 一抗
  • 描述
    兔多克隆抗体to GPT2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GPT2.

  • 阳性对照
    • This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab101876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
  • 组织特异性Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast.
  • 通路Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
  • 序列相似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAT2 antibody
    • Alanine aminotransferase 2 antibody
    • ALAT2_HUMAN antibody
    • ALT2 antibody
    • Glutamate pyruvate transaminase 2 antibody
    • Glutamic alanine transaminase 2 antibody
    • Glutamic pyruvate transaminase (alanine aminotransferase) 2 antibody
    • Glutamic pyruvic transaminase 2 antibody
    • Glutamic--alanine transaminase 2 antibody
    • Glutamic--pyruvic transaminase 2 antibody
    • GPT 2 antibody
    • gpt2 antibody
    see all

Anti-GPT2 antibody 图像

  • All lanes : Anti-GPT2 antibody (ab101876) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab101876 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101876, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

Anti-GPT2 antibody (ab101876)参考文献

ab101876 has not yet been referenced specifically in any publications.

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