Anti-GPT2抗体(ab101876)
Key features and details
- Rabbit polyclonal to GPT2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GPT2抗体
参阅全部 GPT2 一抗 -
描述
兔多克隆抗体to GPT2 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human GPT2 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab123826) -
阳性对照
- This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. -
组织特异性
Expressed at high levels in muscle, adipose tissue, kidney and brain and at lower levels in the liver and breast. -
通路
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
序列相似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. - Information by UniProt
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数据库链接
- Entrez Gene: 84706 Human
- Omim: 138210 Human
- SwissProt: Q8TD30 Human
- Unigene: 460693 Human
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别名
- AAT2 antibody
- Alanine aminotransferase 2 antibody
- ALAT2_HUMAN antibody
see all
图片
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All lanes : Anti-GPT2 antibody (ab101876) at 1/250 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
ICC/IF image of ab101876 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101876, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab101876 尚未被引用在任何文献中。