Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 274-304 of Human GPSN2.
A2058 cell lysate; Human skeletal muscle
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103454 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 36 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Microsomal long and very long chain fatty acid elongation uses malonyl CoA as the 2 carbon donor and consists of 4 sequential reactions. GPSN2 catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA. (Moon and Horton, 2003 [PubMed 12482854]).
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Western blot - Anti-GPSN2 antibody (ab103454)
Anti-GPSN2 antibody (ab103454) at 1/100 dilution + A2058 cell lysate at 35 µg
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPSN2 antibody (ab103454)
ab103454, at 1/50 dilution, staining GPSN2 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"