Anti-GPSM3抗体(ab118118)

概述

  • 产品名称Anti-GPSM3抗体
  • 描述
    小鼠单克隆抗体to GPSM3
  • 经测试应用适用于: WB, ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant full length Human GPSM3 (amino acids 1-160) with a proprietary tag (AAH18724).

  • 阳性对照
    • GPSM3 transfected 293T cell line; GPSM3 Recombinant protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa. This antibody has only been tested against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

靶标

  • 功能Interacts with subunit of G(i) alpha proteins and regulates the activation of G(i) alpha proteins.
  • 组织特异性Expressed in heart, placenta, lung and liver.
  • 序列相似性Contains 3 GoLoco domains.
  • 结构域The GoLoco 1 and/or GoLoco 3 domains exhibit GDI activity towards GDP-bound G(i) alpha protein, but not the GoLoco 2 domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator of G-protein signaling 4 antibody
    • AGS4 antibody
    • C6orf9 antibody
    • G protein signalling modulator 3 (AGS3 like, C. elegans) antibody
    • G-protein signaling modulator 3 (AGS3-like, C. elegans) antibody
    • G-protein-signaling modulator 3 antibody
    • G18 antibody
    • G18.1a antibody
    • G18.1b antibody
    • G18.2 antibody
    • Gpsm3 antibody
    • GPSM3_HUMAN antibody
    • NG1 antibody
    • OTTHUMP00000029351 antibody
    • Protein G18 antibody
    see all

Anti-GPSM3 antibody 图像

  • Anti-GPSM3 antibody (ab118118) at 5 µg/ml + Recombinant protein at 0.2 µg

    Predicted band size : 18 kDa
  • All lanes : Anti-GPSM3 antibody (ab118118) at 5 µg/ml

    Lane 1 : 293T cell line, transfected with GPSM3
    Lane 2 : 293T cell line, non transfected

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 18 kDa
  • Detection limit for recombinant tagged GPSM3 is 0.3 ng/ml as a capture antibody.

Anti-GPSM3 antibody (ab118118)参考文献

ab118118 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (13.5% gel)
Sample Mouse Tissue lysate - whole (Thymus, Spleen, Blood)
Specification Thymus, Spleen, Blood
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"