• 产品名称Anti-GPSM2抗体
    参阅全部 GPSM2 一抗
  • 描述
    兔多克隆抗体to GPSM2
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (YFYLHDYAKA LEYHHHDLTL ARTIGDQLGE AKASGNLGNT LKVLGNFDEA) of Human GPSM2 (NP_037428).

  • 阳性对照
    • Human fetal liver lysate.



Our Abpromise guarantee covers the use of ab84571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.


  • 功能Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
  • 序列相似性Belongs to the GPSM family.
    Contains 4 GoLoco domains.
    Contains 8 TPR repeats.
  • 细胞定位Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DFNB82 antibody
    • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
    • G protein signalling modulator 2 antibody
    • G-protein-signaling modulator 2 antibody
    • Gpsm2 antibody
    • GPSM2_HUMAN antibody
    • HGNC:29501 antibody
    • LGN antibody
    • LGN protein antibody
    • Mosaic protein LGN antibody
    • Pins antibody
    see all

Anti-GPSM2 antibody 图像

  • ab84571 staining GPSM2 in Dog kidney cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C.

    See Abreview

  • ab84571 at 5 µg/ml staining GPSM2 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-GPSM2 antibody (ab84571) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab84571 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab84571 at 5 µg/ml staining GPSM2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-GPSM2 antibody (ab84571)参考文献

ab84571 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3%
Sample Dog Cell (kidney)
Specification kidney
Permeabilization Yes - Triton 0,5%
Fixative Paraformaldehyde

Dr. Inma Banon

Verified customer

提交于 Oct 11 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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I would expect ab31850 to give clearer results in WB, even once the many isoforms are taken into consideration. I would be happy to offer you a free of charge replacement, credit, or refund for this antibody as we...

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For the Spastin antibody ab31850, could you please provide an image of your blot or let me know what size bands you are observing? Spastin has several known isoforms that could account for multiple bands between 54-6...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse Embryo)
Specification Mouse Embryo
Fixative Paraformaldehyde
Permeabilization Yes - Saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

提交于 Mar 14 2012

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