The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 32 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Expression is highest in the periphery, particularly in the stomach, but also in the kidney, liver, pancreas, and prostate. In brain, levels of expression are generally lower than in the periphery, with the exception of cerebellum, spinal cord, and dorsal root ganglia (DRG).
Belongs to the G-protein coupled receptor 3 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cell membrane. Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
ab115,616 at 3-32µg/ml, staining GPRC5C in Formalin-fixed, Paraffin-embedded Human pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.