概述

  • 产品名称Anti-GPR2/CCR10抗体
    参阅全部 GPR2/CCR10 一抗
  • 描述
    山羊多克隆抗体to GPR2/CCR10
  • 特异性Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
  • 经测试应用适用于: WB, ICC/IF, ELISA, ICC, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    VSWGHYSGDEEDAYSAEPLPELC

    , corresponding to amino acids 9-31 of Human CCR10.

  • 常规说明


    Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液10 mM KHPO4, 140 mM NaCl, pH 7.2.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab3904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Can be blocked with GPR2/CCR10 peptide (ab182115).
ICC/IF Use at an assay dependent concentration.
ELISA 1/75000.
ICC 1/1000.
Flow Cyt 1/25.

See Eksteen et al; Von Luttichau et al; Notohamiprodjo et al; Hirata et al and Hislop et al.



IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval at pH 6.0 or pH 8.0; steam for 20 min followed by cooling for 20 min.

靶标

  • 功能Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.
  • 组织特异性Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C C chemokine receptor type 10 antibody
    • C-C chemokine receptor type 10 antibody
    • C-C CKR-10 antibody
    • CC chemokine receptor 10 antibody
    • CC CKR 10 antibody
    • CC-CKR-10 antibody
    • CCR-10 antibody
    • CCR10 antibody
    • CCR10_HUMAN antibody
    • Chemokine (C C motif) receptor 10 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 2 antibody
    • GPR2 antibody
    see all

Anti-GPR2/CCR10 antibody 图像

  • Immunohistochemistry on paraffin sections of human spleen.
  • After retroviral transduction, CCR10-B16 cells were lysed, and CCR10 was detected by Western blotting after gel electrophoresis. A single band representing CCR10 was detected at 42 kD.
  • ab3904, at a dilution of 1/250, staining CCR10 in human tonsil section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, staining GPR2/CCR10 with ab3904.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A biotinylated rabbit anti-goat polyclonal IgG (10 µg/ml) was used as the secondary antibody.

    See Abreview

Anti-GPR2/CCR10 antibody (ab3904)参考文献

This product has been referenced in:
  • Pinto S  et al. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer 14:118 (2014). ICC . Read more (PubMed: 24559071) »
  • Chamberlain G  et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt ; Human, Mouse . Read more (PubMed: 18698345) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (human serum samples)
Specification human serum samples
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

提交于 Jul 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (keratinocytes)
Specification keratinocytes
Preparation Cell harvesting/tissue preparation method: cells were isolated from skin biopsie followed by cell culture
Sample buffer: PBS/10% human IgG
Fixation Paraformaldehyde
Permeabilization Yes - 0,1% Tritonx-100/PBS
Gating Strategy no gate
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Abcam user community

Verified customer

提交于 Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Keratinocytes)
Loading amount 30 µg
Specification Keratinocytes
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization Yes - 0,1% Saponin/PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 06 2013

Thank you for your reply. A quotation has been sent to you in a separate email, the proforma reference number is : *****. DISCOUNT CODE for ab1667 to be tested in Flow Cytometry : ******** Expiration date: DD MM YYYY I am very pleased to hear you would...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Keratinocytes)
Specification Keratinocytes
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 03 2012

Thank you for your enquiry. I am sorry we have no conjugated antibodies available raised against CCR10. There are two primary antibodies that would be suitable for your application, ab3904 and ab1658. You could perhaps use a conjugated secondary with o...

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Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. I have read your customer's technical questionnaire and I have a few comments. Please can you ask your customer to comment on the...

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Thank you for your enquiry. I have obtained a Western blot image and added it to the datasheet for the antibody. I have included a link to the datasheet at the bottom of this email. This link is from the following paper: Murakami T, Cardones AR,...

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Thank you for your enquiry. The starting dilution recommended for this antibody is 1/250. Other than that, you should be able to do a standard Western blot protocol (such as the one listed on our site at http://www.abcam.com/index.html?pageconfig=v...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"