Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 126-155 of Human GPR180.
- WiDr, Y79, 293, A2058 and A375 cell lysates; Human brain tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103453 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500. Predicted molecular weight: 49 kDa.
||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- G protein coupled receptor 180 antibody
- GP180_HUMAN antibody
- GPR 180 antibody
Western blot - GPR180 antibody (ab103453)
All lanes : Anti-GPR180 antibody (ab103453) at 1/100 dilution
Lane 1 : WiDr cell lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A2058 cell lysate
Lane 5 : A375 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GPR180 antibody (ab103453)
ab103453, at 1/50 dilution, staining GPR180 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"