概述

  • 产品名称Anti-GPR161抗体
    参阅全部 GPR161 一抗
  • 描述
    兔多克隆抗体to GPR161
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: MSLNSSLSCR KELSNLTEEE GGEGGVIITQ FIAIIVITIF VCLGNLVIVV, corresponding to N terminal amino acids 1-50 of Human GPR161

  • 阳性对照
    • HepG2 cell lysate or human kidney tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-GPR161 antibody 图像

  • ICC/IF image of ab58679 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58679 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GPR161 antibody (ab58679) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPR161 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-GPR161 antibody (ab58679)参考文献

This product has been referenced in:
  • Feigin ME  et al. G-protein-coupled receptor GPR161 is overexpressed in breast cancer and is a promoter of cell proliferation and invasion. Proc Natl Acad Sci U S A 111:4191-6 (2014). Human . Read more (PubMed: 24599592) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Mouse Cell (NIH3T3 cells)
Specification NIH3T3 cells
Permeabilization Yes - 0.2% Tween 20
Fixative Paraformaldehyde
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Dr. Xuecai Ge

Verified customer

提交于 Aug 09 2013

Thank you very much for your interest in ab58679.

To our knowledge, ab58679 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab58679 now, test it in Flow Cytometryand submit feedback to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"