概述

  • 产品名称
  • 描述
    兔多克隆抗体to GPM6B
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human GPM6B.

  • 阳性对照
    • This antibody gave a positive signal in Mouse cerebellum tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab92988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能
    May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.
  • 组织特异性
    Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia.
  • 序列相似性
    Belongs to the myelin proteolipid protein family.
  • 细胞定位
    Cell membrane. Colocalizes with SERT at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • glycoprotein M6B antibody
    • GPM6B antibody
    • GPM6B_HUMAN antibody
    • M6b antibody
    • Neuronal membrane glycoprotein M6-b antibody
    • Neuronal membrane glycoprotein M6b antibody
    • protolipid M6B antibody
    • rhombex 29 antibody
    see all

图片

  • Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Choi KM  et al. Distribution of the Immunoreactivity for Glycoprotein M6B in the Neurogenic Niche and Reactive Glia in the Injury Penumbra Following Traumatic Brain Injury in Mice. Exp Neurobiol 22:277-82 (2013). IHC . Read more (PubMed: 24465143) »

See 1 Publication for this product

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