Anti-GPM6B抗体(ab92988)

概述

  • 产品名称Anti-GPM6B抗体
    参阅全部 GPM6B 一抗
  • 描述
    兔多克隆抗体to GPM6B
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human GPM6B.

  • 阳性对照
    • This antibody gave a positive signal in Mouse cerebellum tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab92988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.
  • 组织特异性Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia.
  • 序列相似性Belongs to the myelin proteolipid protein family.
  • 细胞定位Cell membrane. Colocalizes with SERT at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • glycoprotein M6B antibody
    • GPM6B antibody
    • GPM6B_HUMAN antibody
    • M6b antibody
    • Neuronal membrane glycoprotein M6-b antibody
    • Neuronal membrane glycoprotein M6b antibody
    • protolipid M6B antibody
    • rhombex 29 antibody
    see all

Anti-GPM6B antibody 图像

  • Anti-GPM6B antibody (ab92988) at 1/250 dilution + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab92988 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92988 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPM6B antibody (ab92988)参考文献

ab92988 has not yet been referenced specifically in any publications.

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