概述
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产品名称Anti-GPLD1抗体
参阅全部 GPLD1 一抗 -
描述兔多克隆抗体to GPLD1
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human GPLD1.
(Peptide available as ab92422.)
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阳性对照
- This antibody gave a positive signal in Human and Mouse Plasma Total Protein Preparations.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab82330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
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序列相似性Belongs to the GPLD1 family.
Contains 7 FG-GAP repeats. -
细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 2822 Human
- Entrez Gene: 14756 Mouse
- Entrez Gene: 291132 Rat
- Omim: 602515 Human
- SwissProt: P80108 Human
- SwissProt: O70362 Mouse
- SwissProt: Q8R2H5 Rat
- Unigene: 533291 Human
see all -
别名
- Glycoprotein phospholipase D antibody
- Glycosyl-phosphatidylinositol-specific phospholipase D antibody
- GPI-PLD antibody
see all
图片
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Western blot - Anti-GPLD1 antibody (ab82330)All lanes : Anti-GPLD1 antibody (ab82330) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : Mouse Plasma Total Protein Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
The GPLD1 protein has a predicted molecular weight of 92 kDa, however it has several potential glycosylation sites (SwissProt data) that might explain its migration at a higher MW. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
文献
ab82330 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"